Midterm2_1

Midterm2_1 - BMB100B Winter 2008 Midterm #2 Name:_ Name:...

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BMB100B Winter 2008 Midterm #2 Name:____________________________ Name: T.A. Name: You have 100 minutes to complete the exam. Write your name on every page. Show all work where appropriate. You may use a calculator and pen/pencil but no other materials. Use the back of pages and the final blank page for scratch space. Make sure your final answers are clearly indicated. Problem 1 ___________ / 12 pts Problem 2 ___________ / 20 pts Problem 3 ___________ / 28 pts Problem 4 ___________ / 16 pts Problem 5 ___________ / 24 pts __________________________________________ Total ___________ / 100 pts ___________________________________________________________________________ Page 1
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BMB100B Winter 2008 Midterm #2 Name:____________________________ 1) In the serine phosphorylation reaction catalyzed by Cyclin-dependent kinase, the serine is activated as a nucleophile through deprotonation by Asp127. a) Do you anticipate that the pKa of Asp127 in Cdk is higher or lower than the pKa of free aspartic acid in water? Explain. [8 pts] b) What amino acid types would you anticipate are nearby in the structure to give rise to this pKa shift? Explain. [4 pts] Page 2
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BMB100B Winter 2008
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Midterm2_1 - BMB100B Winter 2008 Midterm #2 Name:_ Name:...

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