Chapter 16

Chapter 16 - Chapter 16 I. Evidence that DNA is genetic...

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Chapter 16 I. Evidence that DNA is genetic material A. Mendel => heritable factors B. Morgan => genes on chromosomes C. In 1928, Griffith working with Streptococcus pneumonia demonstrated that genetic material could be transferred from one bacterium to another 1. The trait from the smooth cells was transferred to the rough cells, and the rough cells were transformed 2. Transformation – the assimilation of external genetic material by a cell – confirm new traits to an organism (did not know DNA is the genetic materials at this time) II. More evidence that DNA is the genetic material came from the study of bacteriophages A. Bacteriophages (a.k.a. phage) – a virus that infects bacteria B. Hershey and Chase - if something’s transferred – it should be protein or DNA 1. Labeled phage coat (protein) with 35 - chose 35S b/c there’s sulfur in amino acids 2. Looked to see if bacterial cells became radioactive (with 35S) Does not contain a lot of radioactive - thought maybe 3. Then, labeled DNA with 32P No P in amino acids A lot of 32 P was transferred – contain a lot of radioactive 4. Concluded that the DNA was the genetic material in a virus Use virus to infect bacteria BUT WHAT ABOUT EUKARYOTES? C. Circumstantial Evidence
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Chapter 16 - Chapter 16 I. Evidence that DNA is genetic...

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