Lecture 6 - Jan 27, 2010

Lecture 6 - Jan 27, 2010 - DNA Polymerase Structure No DNA...

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DNA Polymerase Structure
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No DNA Forms without a Primer DNA Polymerases Need a primer to start working Unidirectional 5’ – 3’ Many kinds in a cell DNA Pol III: elongation DNA Pol I: primer removal DNA Pol II: repair
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DNA Replication: Mechanism Leading strand Lagging strand
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DNA Replication: Two polymerases are stay together
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Primer removal and ligation
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DNA Replication Processive catalyis
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Animation 11.3 DNA Replication, Part 2: Coordination of Leading and Lagging Strand Synthesis
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3D Animations of DNA replication http://www.youtube.com/watch?v=nIwu5MevZyg&feature=related
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CLICKER QUESTION Which of the following statements about the replication of DNA is false ? a. New nucleotides are added to the most 3′ end of one strand and the most 5′ end of the other strand. b. Replication occurs at a very large protein complex called the replication complex. c. Replication requires synthesis of a short RNA primer to provide a free 3′ end. d. Replication can proceed in two directions at once.
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CLICKER QUESTION Integrate what you learned in Chapter 9 and chapter 11: At the end of DNA replication but before cell division, a human cell contains
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Lecture 6 - Jan 27, 2010 - DNA Polymerase Structure No DNA...

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