HWset2W10 - BIOL/MCBL 121 Homework Set#2 Winter 2010 1)...

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BIOL/MCBL 121 Homework Set#2 Winter 2010 1) Differentiate between transformation, transduction and conjugation as methods to introduce DNA into bacterial cells. Which method(s) are most relevant in nature? 2) a) Describe the mechanism of conjugation. What is the difference between F - , F + and Hfr cells? How do these mating types relate to the F plasmid? b) For what purpose is an interrupted mating test used in bacterial genetics? 3) An Hfr E. coli contains the following genetic traits: lac + , kan s , his + , leu + , str r . It is mated with a recipient E. coli that contains the opposite genetic traits. Note that kan S means the absence of the kanamycin resistance gene in the Hfr strain, which will allow growth only of the recipient cells in the presence of kanamycin. This is necessary for marking the transfer of the Hfr traits to the recipient strain. At periodic intervals, conjugation is disrupted and cells are plated onto several types of defined media. Nothing grows at the beginning of the experiment on media containing lactose,
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This note was uploaded on 03/18/2010 for the course BIOL biol121 taught by Professor Dsaofj during the Spring '10 term at UC Riverside.

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HWset2W10 - BIOL/MCBL 121 Homework Set#2 Winter 2010 1)...

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