lecture 6 W10final

lecture 6 W10final - 1/14/10 Lecture 6 Environmental...

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1/14/10 1 Lecture 6 Environmental Influences and Control of Microbial Growth “Normal” growth conditions are from 20° C to 40°C, neutral pH, and 0.9% salt concentration Any organism that can grow outside of that range is considered an extremophile Microbiology: An Evolving Science © 2009 W. W. Norton & Company, Inc. 2
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1/14/10 2 Microbiology: An Evolving Science © 2009 W. W. Norton & Company, Inc. 3 Environmental Limits on Microbial Growth Temperature pH Osmolarity Oxygen Pressure 4 Assaying Environmental Influences DNA Microarray-gene expression Two-Dimensional Gel-protein expression
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1/14/10 3 DNA Microarray [INSERT FIGURE 8.9] 5 Red = Condition A; Green= Condition B 6
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1/14/10 4 DNA Microarray Consist of molecules of single-stranded DNA (ssDNA) immobilized on glass slides or silicon chips- each representing a single gene in the genome Single-stranded, fluorescently labeled DNA washed over an array will adhere only at locations on the array where there are complementary DNA sequences Variety of scientific uses of DNA microarrays Monitoring gene expression Diagnosis of infection Identification of organisms in an environmental sample 7 DNA Microarray Diagnosis of infection Microarray made with the sequences of many different pathogens In 2003 DNA Microarray containing 12,000 unique sequences revealed that SARS was a Coronavirus Identification of organisms in an environmental sample Microarray used to monitor the presence or absence of a microbe 8
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1/14/10 5 Two-Dimensional Gel Separation via two methods Isoelectric point Molecular weight Protein expressed at: Pink : pH 4.9 Green : pH 7.0 9 Microbiology: An Evolving Science © 2009 W. W. Norton & Company, Inc. 10 Changes in Temperature Growth rate increases with temperature Proteins denature if temperature too high Microbial proteins adapted to temperature range: Psychrophiles Cold: O°C–20°C Mesophiles 12°C–45°C Thermophiles 40°C–80°C Extreme thermophiles 65°C–113°C
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1/14/10 6 Microbiology: An Evolving Science © 2009 W. W. Norton & Company, Inc. 11
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lecture 6 W10final - 1/14/10 Lecture 6 Environmental...

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