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Unformatted text preview: DNA. The enzyme reads from the 5 ′ end of DNA. Note that the two strands of DNA are complementary so you will have staggered (or sticky) ends after your cuts. Cut the plasmid and gene. Although enzymes work by trial-and-error to find their substrate, the arrows ( ↑ ) will help you locate the correct sequence. Ligation. You can use tape in place of the DNA ligase that covalently joins pieces of DNA. Match the complementary bases of the staggered ends to tape the gene into the plasmid. Voilà! A recombinant plasmid to insert into a cell. Questions: How will you identify cells carrying the recombinant plasmid? Cells carrying the original plasmid? Cells without a plasmid?...
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This note was uploaded on 03/18/2010 for the course BIOL 240 taught by Professor Staff during the Spring '08 term at Skyline College.
- Spring '08