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biotechhandout - plasmid vector Semi-synthetic products...

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Biotechnology Skyline College 1 Biology 240—Case A Problem Your lipase-producing strain grows slowly in a small-scale fermenter. Yield of lipase, a primary metabolite, is low. How can you improve the yield? Objectives 1. Compare and contrast each of the following pairs: a. Natural selection and artificial selection b. Selective breeding and recombinant DNA 2. Explain how each of the following is used to improve a product: selection; high-throughput screening; enrichment; mutation; directed evolution. 3. Describe the process of making rDNA using a
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Unformatted text preview: plasmid vector. Semi-synthetic products ________ ________ ________ Biotechnology Skyline College 2 Biology 240—Case Value added Enrichment Evolution Natural Selection • • • Artificial Selection • • • • Biotechnology Skyline College 3 Biology 240—Case _____________________ Translation Directed Evolution • Goal is achieved • • • Selection & Screening • Select strains • • • • • • • • Select mutants … Scale-up...
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