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Materials&methods - Materials Methods Daphnia...

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Materials/ Methods Daphnia species D. Pulex was studied to see how different concentration levels of KCL affected the organisms. A microscope was used to view the daphnia in a slide with a well to observe their behavior. After carefully observing, the activities that were selected to measure were the heartbeat per 30 seconds and the circular movement per one minute. (Explain more on why we choose these activities) In order to test the heartbeat a baseline had to be measures by measuring the heartbeat per 30 seconds of ten organisms. A plastic pipette is used to move the D. Pulex from the cup into a slide with a well. The organisms need water in order to survive but do not fill the well with water otherwise the organism will move too much causing the heartbeat to be difficult to measure. Use a timer and a counter to accurately obtain the heartbeat measurement. Next, put the ten organisms in a 250 ppm KCL solution and let them sit in the solution for 15 minutes.
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