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methods for Bio lab III

methods for Bio lab III - Cont’d • The liquid is then...

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3/19/10 Methods Standard curve for protein is taken from the Fall Quarter experiment while the carbohydrate standard curve is made. Equal masses of protein & chocolate bars are obtained and soaked in water a week before experiment. The soaked bars are then grind up in potassium buffers while keeping record of the volume added. The suspension is then poured into the cheese cloth and the excess is squezed in the beaker.
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3/19/10
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Unformatted text preview: 3/19/10 Cont’d • The liquid is then transferred into 15mL centrifuge and centrifuged for 3 minutes at 3000rpm • About 10mL of the supernatant is pipetted. • The protein & Carbohydrate of the extract are measured. • A 1:10 dilution is made for each extract before the protein/carb assay is done. ( you could say the 100microL of extract + 900microL of water in centrifuge tube)...
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