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Unformatted text preview: 3/19/10 Cont’d • The liquid is then transferred into 15mL centrifuge and centrifuged for 3 minutes at 3000rpm • About 10mL of the supernatant is pipetted. • The protein & Carbohydrate of the extract are measured. • A 1:10 dilution is made for each extract before the protein/carb assay is done. ( you could say the 100microL of extract + 900microL of water in centrifuge tube)...
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- Spring '10
- ade ach xtract, tandard curvefor prote, fore prote in/carb, pipe d. tte, Thesuspe nsion