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protocol week 7 - Group XX3 Jacqualyn Schulman Jaclyn...

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Group : XX 3 Jacqualyn Schulman Jaclyn Fingerhut Norkhairin Yusuf Protocol for Week 7 *Since the absorbancy result for the Carbohydrate Standard Curve was too high because the solution was too dark from last week, a higher diluted solution is made by adding more distilled water for this week. 1. Prepare a carbohydrate standard curve a. Using the same procedure from week 6 protocol but with different amount of distilled water in microliters. Cuvettes Blank 1 2 3 4 5 Distilled Water 5000 4000 3000 2000 1000 0 D-Glucose solution 0 100 200 300 400 500 2. Making an aqueous extract of Power Bar, Cliff Bar and a cookie The same procedure is used from last week. 3. Measuring the protein concentrations of the samples. For Power Bar, Cliff Bar and the cookie a. A 1:10 dilution is made for both bar’s extract before beginning the protein assays. (100 microliters of extract + 900 microliters of water) b. 7 cuvettes are labeled and alliquoted like in the table below.
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