2000 - UNIVERSITY OF HONG KONG BACHELOR OF SCIENCE FINAL...

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Unformatted text preview: UNIVERSITY OF HONG KONG BACHELOR OF SCIENCE: FINAL EXAMINATION BOTANY/ECOLOGY & BIODIVERSITY/ZOOLOGY: BIOL1106 Genetics 26 May, 2000 9:30 am — 11:30 am Candidates may use any self-contained, silent, battery-operated and pocket-sized calculator. The calculator should have numeral-display facilities only and should be used only for the purpose of calculation. It is the candidate's responsibility to ensure that his/her calculator operates satisfactorily. Candidates must record the name and type of their calculators on the front page of their examination scripts. Answer any FIVE questions. All questions carry equal marks. 1. Purple anthocyanin pigment in tomato stems is governed by a dominant gene A, and its recessive allele a produces green stem. Hairy stem is g0verned by a dominant gene H, and hairless stem by its recessive allele h. A dihybrid purple, hairy plant is crossed with a green, hairless plant and produces 73 purple, hairy: 12 purple, hairless: 75 green, hairless: 9 green, hairy. (a) Is the F1 purple: green ratio compatible with the expectation for alleles? Show your analysis. (b) Test the F I data for independent assortment. What conclusion do you reach? A dominant gene in corn (Kn) results in the proliferation of vascular tissues in a trait called "knotted leaf“. A heterozygous knotted leaf plant is crossed to a normal plant producing 153 knotted leaf and 178 normal progeny. (a) Test your hypothesis using Chi-square method. (b) Apply Yates' correction in the calculation of Chi-square. Are these results consistent with your hypothesis? The numbers of genotypes at a locus are as follows: Genotype AA Aa aa Observed numbers 25 55 20 (a) Calculate the frequency of each allele. (b) Calculate the expected frequency for each genotype. (c) Calculate the expected number for each genotype. (d) Test for Hardy-Weinberg equilibrium. The critical Chi-square value at the 95% significance level is 3.841. Describe the distinctive morphological features of the metaphase chromosomes. Discuss the significance of chromosome number. Describe the structural features of DNA and discuss why DNA can serve as a genetic material. Assuming that the Pacific salmon breed mainly in two rivers, the Fraser River in Canada and the Columbia River in the United States, a fish biologist investigated the relative contribution of the two rivers to the common stock in the Pacific. He took a sample of the Pacific salmon from each of the two rivers as well as a sample from the common stock in the Pacific Ocean, and carried out protein electrophoresis on one enzyme locus. The allele frequency data from each of the three samples are summarized in the following table. What is the relative contribution of the two rivers to the common salmon stock in the Pacific? Fraser River Columbia River Pacific Ocean P 0.1 0.8 0.7 Q 0.9 0.2 0.3- The probublllfles of exceeding amen.» chi-square values for degree: of freedom from 1 to 50 when the expected hypoihesis is true‘ cuuaueu-~§ NNNN‘eh— M‘kkNNQ 20 25 30 50 Probabilth .95 .90 . 70 .50 .30 .20 .10 .05 .01 .00] .004 .016 .15 .46 1.07 1.64 2.71 3.84 6.64 10.83 .10 .21 .71 1.39 2.41 3.22 4.61 5.99 9.21 13.112 .35 .58 1.42 2.37 3.67 4.64 6.25 7.82 11.35 16.27 .71 1.06 2.20 3.36 4.88 5.99 7.78 9.49 13.28 18.47 1.15 1.61 3.00 4.35 6.06 7.29 9.24 11.07 15.09 20.52 1.64 2.20 3.83 5.35 7.2.3 8.56 10.65 12.59 16.81 22.46 2.17 2.83 4.67 6.35 8.38 9.80 12.02 14.07 18.48 24.32 2.73 3.49 5.53 7.34 9.52 11.03 13.36 15.51 20.09 26.13 3.33 4.17 6.39 8.34 10.66 12.24 14.68 16.92 21.67 27.88 3.94 4.87 7.27 9.34 11.78 13.44 15.99 18.31 23.21 29.59 4.58 5.58 8.15 10.34 12.90 14.63 17.28 19.68 24.73 31.26 5.23 6.30 9.03 11.34 14.01 15.81 18.55 21.03 26.22 32.91 5.89 7.04 9.93 12.34 15.12 16.99 19.81 22.36 27.69 34.53 6.57 7.79 10.82 13.34 16.22 18.15 21.06 23.69 29.14 36.12 7.26 8.55 1 1.72 14.34 17.32 19.31 22.31 25.00 30.58 37.70 10.85 12.44 16.27 19.34 22.78 25.04 28.41 31.41 37.57 45.32 14.61 16.47 20.87 24.34 28.17 30.68 34.38 37.65 44.31 52.62 18.49 20.60 25.51 29.34 33.53 36.25 40.26 43.77 50.89 59.70 34.76 37.69 44.31 49.34 54.72 58.16 63.17 67.51 76.15 86.66 do not reject i reject a1 .05 level ‘ Abridged lion Table IV 01 Fisher and Yates, 51:01:15“! fable: for link: ‘ A ' ' ‘ ' ' of m. “who” and “whim”. 31:51, wwbural mdMuFullmdl. 01".! an! lord List. Edmbwgh, by pemmnen -- END OF PAPER -- ...
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