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splicing and processing of RNA

splicing and processing of RNA - BIOC2603 Principles of...

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BIOC2603 Principles of Molecular Genetics Splicing and RNA processing RNA editing Gene evolution: exons and introns Dr. Mai Har Sham Department of Biochemistry
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Advanced References Watson et al. (2004) Molecular Biology of the Gene. 5 th Ed. Pearson/ Benjamin Cummings Publ. Co. Bass BL (2001) RNA Editing. OUP. Cooper DN (1999) Human gene evolution. Bios Sci. Publ. Stone ED and Schwartz RJ (1990) Intervening sequences in evolution and development. OUP. Stuart KD et al. (2005) Complex management: RNA editing in trypanosomes. Trends in Biochemical Sciences, 30(2): 97-105.
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Splicing and RNA processing Outline Introduction Eukaryotic genes eukaryotic RNAs Processing of eukaryotic mRNA Mechanisms of mRNA intron splicing Spliceosome Alternative splicing
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of mRNA prokaryotic mRNA polycistronic usually can be translated immediately without further modification eukaryotic mRNA monocistronic 5’-cap 3’-poly(A) tail intron splicing
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of eukaryotic mRNA 5’-cap 7-methylguanosine residue joined to 5’ nucleoside via a 5’-5’ triphosphate bridge 3’-poly(A) tail about 100-200 A’s generated from ATP through stepwise action of poly(A) polymerase intron splicing introns are excised and the exons are connected in the splicing process to yield the mature mRNA
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Two reactions involved in intron excision (splicing): Formation of a 2’5’ phosphodiester bond between A (~20 to 50 residues upstream of 3’ splice site) and the phosphate of G (5’ splice site)
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