splicing and processing of RNA

Splicing and - BIOC2603 Principles of Molecular Genetics Splicing and RNA processing RNA editing Gene evolution exons and introns Dr Mai Har Sham

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BIOC2603 Principles of Molecular Genetics ± Splicing and RNA processing ± RNA editing ± Gene evolution: exons and introns Dr. Mai Har Sham Department of Biochemistry
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Advanced References ± Watson et al. (2004) Molecular Biology of the Gene. 5 th Ed. Pearson/ Benjamin Cummings Publ. Co. ± Bass BL (2001) RNA Editing. OUP. ± Cooper DN (1999) Human gene evolution. Bios Sci. Publ. ± Stone ED and Schwartz RJ (1990) Intervening sequences in evolution and development. OUP. ± Stuart KD et al. (2005) Complex management: RNA editing in trypanosomes. Trends in Biochemical Sciences, 30(2): 97-105.
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Splicing and RNA processing Outline ± Introduction ± Eukaryotic genes ± eukaryotic RNAs ± Processing of eukaryotic mRNA ± Mechanisms of mRNA intron splicing ± Spliceosome ± Alternative splicing
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of mRNA ± prokaryotic mRNA ± polycistronic ± usually can be translated immediately without further modification ± eukaryotic mRNA ± monocistronic ± 5’-cap ± 3’-poly(A) tail ± intron splicing
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of eukaryotic mRNA ± 5’-cap ± 7-methylguanosine residue joined to 5’ nucleoside via a 5’-5’ triphosphate bridge ± 3’-poly(A) tail ± about 100-200 A’s generated from ATP through stepwise action of poly(A) polymerase ± intron splicing ± introns are excised and the exons are connected in the splicing process to yield the mature mRNA
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Two reactions involved in intron excision (splicing): ± Formation of a 2’5’ phosphodiester bond between A (~20 to 50 residues upstream of
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This note was uploaded on 03/19/2010 for the course BIOC BIOC2603 taught by Professor Dr.mhsham during the Spring '07 term at HKU.

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Splicing and - BIOC2603 Principles of Molecular Genetics Splicing and RNA processing RNA editing Gene evolution exons and introns Dr Mai Har Sham

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