LAB 4 - Photosynthesis

LAB 4 - Photosynthesis - (not absorbed) Chlorophyll a green...

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LAB 4 - Photosynthesis 6H O + 6CO + light E + pigments C H O + 6O + evaporated H O * unusable sunlight energy converted into usable chemical energy
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Pea Leaf Stoma
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• Light-dependent or Light Reactions ( grana ) • Light-independent or Dark Reactions ( stroma )
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• splitting of H 2 O and production of O 2 , ATP and NADPH Light reactions • CO 2 reacts with ribulose bisphosphate (RuBP), a 5-C chemical, to form glucose ATP and NADPH used in the process Dark reactions – Calvin cycle
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Activity #4 – Chlorophyll and Photosynthesis 1. Variegated Coleus leaves 2. Corn ( Zea mays ) seedlings – green and albino Benedict’s test Iodine test
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Chlorophyll a and Accessory Pigments A pigment – any substance that absorbs light The color of the pigment comes from the wavelengths of light reflected
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Unformatted text preview: (not absorbed) Chlorophyll a green pigment Accessory pigments absorb energy that Chl a does not absorb Chl b , xanthophylls, carotenoids ( carotene), anthocyanins Chlorophyll a Chlorophyll looks green because it absorbs red and blue light. Activity #5 Paper Chromatography Chloroplast pigments separated (extracted) by their solubility in solvents molecular size and polarity of pigments matter the more soluble (non-polar) the component the further it goes Each pigment has a specific R f ( R atio of f ronts or R etardation f actor) R f value depends on the temperature , type of solvent and type of paper (acetone & petroleum ether) (matrix) Write ups P90 problem 4 and 5...
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LAB 4 - Photosynthesis - (not absorbed) Chlorophyll a green...

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