Lecture 13 - Nano-Bio imaging 2

Lecture 13 - Nano-Bio imaging 2 - Positron Emission...

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Unformatted text preview: Positron Emission Tomography Annihilation 511 keV 511 keV Positron Electron PET isotopes: 18 F, 64 Cu, 86 Y, 68 Ga, 124 I, etc. PET Agent 89 Zr 86 Y 76 Br Isotope 3.3 d 14.7 h 16.2 h Half-life 68 Ga 15 O 13 N Isotope 68 min 2 min 10 min Half-life 26.0 h 72 As 12.8 h 64 Cu 4.2 d 124 I 1.8 h 18 F 53 min 94m Tc 20 min 11 C Half-life Isotope Half-life Isotope Functionalization of SWNT Sidewall Non-covalent Endohedral π Stacking SWNT Defect O O O n Phospholipid PEG HN HN NH NH N H O O O O O H N NH 2 NH O HO HO N H c(RGDyK) N N N N CO 2 H HO 2 C O CO 2 H O O O n DOTA RGD- & DOTA-Conjugated SWNT Target α v β 3 PET SWNT Liu, Cai et al., Nat Nanotechnol, 2007. 64 Cu 64 Cu 64 Cu-2K-NT-RGD 64 Cu-5.4K-NT-RGD 0.5 h 6 h 18 h 20 %ID/g 0 %ID/g RGD Conjugation Improves Tumor Uptake Liu, Cai et al., Nat Nanotechnol, 2007. 10 20 30 40 B l o o d S k i n M u s c l e I n t e s t i n e H e a r t L u n g L i v e r K i d n e y S p l e e n S t o m a c h B o n e P a n c r e a s U 8 7 M G B r a i n %ID/g SWNT PEG2000 RGD SWNT PEG5400 RGD 64 Cu-2K-NT-RGD 64 Cu-5.4K-NT-RGD 10 20 30 40 B l o o d S k i n M u s c l e I n t e s t i n e H e a r t L u n g L i v e r K i d n e y S p l e e n S t o m a c h B o n e P a n c r e a s U 8 7 M G B r a i n %ID/g SWNT PEG2000 RGD SWNT PEG5400 RGD 64 Cu-2K-NT-RGD 64 Cu-5.4K-NT-RGD Biodistribution at 24 h after Injection Liu, Cai et al., Nat Nanotechnol, 2007. 0.5 h 6 h 18 h 20 %ID/g 0 %ID/g Blocking Experiment & α v β 3-Negative Tumor Blocking with 15 mg/kg c(RGDyK) 64 Cu-5.4K-NT-RGD Control Tumor Liu, Cai et al., Nat Nanotechnol, 2007. Are We Truly Imaging SWNT? 511 keV Raman Spectroscopy of SWNT Raman Quantification of SWNT 20 %ID/g 0 %ID/g 20 %ID/g 0 %ID/g 20 %ID/g 0 %ID/g Liu, Cai et al., Nat Nanotechnol, 2007. Raman Imaging with SWNT Zavaleta et al., Nano Lett, 2008. Liu et al., J Am Chem Soc, 2008. In Vivo In Vitro Photoacoustic Tomography with SWCNTs De la Zerda et al., Nat Nanotechnol, 2008. MRI with SWNT Hartman et al., Nano Lett, 2008. Near-Infrared Fluorescence Imaging with SWNT Welsher et al., Nano Lett, 2008. Leeuw et al., Nano Lett, 2007. Tumor Cell Living Larva Brightly Fluorescent SWNTs Welsher et al., Nat Nanotechnol, 2009. Non-Covalently Functionalized SWNTs O O O O O P O O O N C O H l-2kPEG: n=45 l-5kPEG: n=115 l-7kPEG: n=160 l-12kPEG: n=230 polyethylene glycol (PEG) O O O n br-7kPEG: O H N 115 O O O 3 O O O O O O 12 1 2 1 2 20000 40000 60000 1400 1600 1800 Raman Shift(cm-1 ) Raman Counts 1 10 100 1000 10000 0.001 0.1 10 [SWNT] (mg/L) Raman Counts (x1000) Liu et al., PNAS, 2008. Circulation Time of SWNTs with Different PEGylations Liu et al., PNAS, 2008. Clearance of SWNT from Mice...
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This note was uploaded on 03/23/2010 for the course MATERIAL S 803 taught by Professor Samuel during the Spring '10 term at University of Wisconsin.

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Lecture 13 - Nano-Bio imaging 2 - Positron Emission...

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