BIO311C_S10_Chapter 6 part 1

BIO311C_S10_Chapter 6 part 1 - Eukaryotic cells Chapter 6...

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Eukaryotic cells Chapter 6
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Endosymbiotic theory
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The Scale of Life http://learn.genetics.utah.edu/content/begin/cells/scale/
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Units of measurement
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Light microscopes first used and perfected in 1600s Phase contrast developed -1940s Electron microscopes developed since 1950s Confocal microscope developed since 1980s Microscopes provide windows to the cell
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Magnification Ratio of an object’s image to its real size Resolution Measure of clarity of image Contrast Visible differences in part of the sample
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Light Microscopes Magnification approx 1000 x Resolution 200 nm Electron Microscopes Magnification approx 250, 000 x Resolution 2 nm
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Dark objects visible against a bright background. Brightfield Illumination Figure 3.4a, b
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Light objects are visible against a dark background Darkfield Illumination Figure 3.4a, b
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Uses fluorochromes and a laser light. The laser illuminates each plane in a specimen to produce a 3-D image. Confocal Microscopy Figure 3.7
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Accentuates diffraction of the light that passes through a specimen. Phase-Contrast Microscopy
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BIO311C_S10_Chapter 6 part 1 - Eukaryotic cells Chapter 6...

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