lecture_10

lecture_10 - BIOPROCESS SIMULATION, ECONOMICS AND DESIGN...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
BIOPROCESS SIMULATION, ECONOMICS AND DESIGN CHARLES L. COONEY DOWNSTREAM PROCESSING COURSE MIT, CAMBRIDGE, MA
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
PROCESS SYNTHESIS & PROCESS ANALYSIS Where do you begin Process Design? Information on Products, raw materials, etc. M&E balances, sizing, costing, economic evaluation
Background image of page 2
YOUR GOAL IF YOU DON’T KNOW WHERE YOU ARE GOING AND YOU DON’T HAVE A MEANS OF MEASURING WHERE YOU ARE THEN YOU WON’T KNOW WHEN YOU ARRIVE
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
STEPS IN PROCESS DESIGN 1. Product definition Product specifications Defines analytical needs Market size 2. Select the synthetic technology 3. Create process flow diagram (PFD) 4. Materials (reagents and consumables) Equipment Utilities Labor 5. Assess assumptions and uncertainty 6. Identify economic and quality hot spots 7. Assess profitability and risk 8. Create the R/D agenda
Background image of page 4
WHAT DO I WANT & NEED TO KNOW FOR PROCESS MODELING AND SIMULATION What is the cost of goods? What are the cost sensitive operating parameters? What are the assumptions and where is the uncertainty? Where are the economic hot spots? What is the impact of process change on cost and quality? Are there alternative processes? Where are the process bottlenecks? PROFIT = VF M ( S P S A C M )
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
WHEN SELECTING UNIT OPERATIONS THERE ARE CHOICES AND DECISIONS MUST BE MADE Cell Harvesting Bioreactor 1. Centrifugation 2. Microfiltration 3. Ultrafiltration Intracellular Products Extracellular Products Biomass Removal 1. Vacuum Filtration 2. Centrifugation 3. Microfiltration 4. Ultrafiltration 5. Press Filtration 6. Candle Filtration 7. Flotation Cell Disruption 1. Homogenization 2. Bead Milling 3. Osmotic Shock Cell Debris Removal 1. Centrifugation 2. Microfiltration 3. Vacuum Filtration 4. Press Filtration Renaturation 1. Solubilization 2. Reoxidation Dehydration or Solvent Removal 1. Spray Drying 2. Freeze Drying 3. Fluid Bed Drying Product Extraction By 1. Organic Solvents 2. Polymer/Polymer 3. Polymer/Salt 4. Supercritical Fluids 5. Adsorption 6. Reverse Micelles 7. Distillation Concentration 1. Ultrafiltration 2. Evaporation 3. Reverse Osmosis 4. Precipitation 5. Crystallization 6. Extraction 6. Adsorption 7. Distillation Final Purification Requirements Contemporary Downstream Processing of Biological Materials Denatured Products Requirements Low Purity High Purity 1. Adsorption 2. Gel Filtration 3. Diafiltration 4. Electrodialysis 5. Electrophoresis Figure by MIT OCW.
Background image of page 6
Overview Bioengineering Multipliers Equipment prices Volume/Mass of Product Process Flow Diagram Conversions, Yields Purchase Equipment Cost Raw Materials Capital Investment Operating Costs Utilities Labor Consumables
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
CASE STUDIES Protein synthesis using mammalian cells for Monoclonal Antibody production Microbial process producing the antibiotic Penicillin Alkaline Protease production by microbial fermentation
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 03/24/2010 for the course ARCH 456 taught by Professor Arch during the Spring '10 term at MIT.

Page1 / 47

lecture_10 - BIOPROCESS SIMULATION, ECONOMICS AND DESIGN...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online