Lecture 5

Lecture 5 - Lecture 5: Light Microscopes 1. Understand the...

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Lecture 5: Light Microscopes 1. Understand the sizes of microorganisms and be able to interconvert the metric units of length 2. Understand the difference between a simple and a compound microscope and between resolution and magnification a. Simple Microscope – a nice magnifying glass b. Compound Microscope – 2 lenses (an objective lens and an eyepiece) c. Resolution = 0.5 X wavelength/aperture (ex: 0.5 x 530 nm/1.25 = 0.2 um i. Resolution is improved by: 1. using short wavelength light 2. Increasing the numerical aperture d. Magnification 3. Know the difference between simple and differential staining and the roles each step plays in the Gram stain a. Fixation – to immobilize cells and preserve aperture i. Heat Fixation: maintains overall cell structure ii. Chemical Fixation: Preserves internal structure b. Staining – to increase visibility and accentuate structures c. Simple staining d. Differential Staining – allows you to differentiate a organism from a group of other organisms
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Lecture 5 - Lecture 5: Light Microscopes 1. Understand the...

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