Lecture 6

Lecture 6 - Lecture 6: Electron Microscopy 1. The key...

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Lecture 6: Electron Microscopy 1. The key differences between light and electron microscope: a. Maximum Magnification i. Light: 1000-1500 ii. Electron: 100,000+ b. Best Resolution i. Light: 0.2 um ii. Electron 0.2 nm c. Radiation Source i. Light: Visible Light ii. Electron: Electron Beam d. Wavelength i. Light: 530nm ii. Electron: 0.005 nm e. Medium of Travel i. Light: Air ii. Electron: High Vacuum f. Type of Lens i. Light: Glass ii. Electron: Electromagnet 2. The various steps in sample preparation for TEM and SEM a. TEM i. Fixation (gluteraldehyde) ii. Dehydration (ethanol) – so that the water doesn’t boil and mess up the microscope iii. Embedding (in plastic) iv. Sectioning into very thin slices (thin enough to be able to pass electrons through)
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v. Staining (heavy metals) – because when bombarded with electrons the heavy metals absorb them rather than letting them pass through vi. Bombard with high energy electrons b. SEM i. Fixation (gluteraldehyde) ii. Dehydration (ethanol) iii. Sputter Coating (evaporate a metal in an enclosed chamber with the sample to
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This note was uploaded on 03/25/2010 for the course MB 351 taught by Professor Keen during the Spring '08 term at N.C. State.

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Lecture 6 - Lecture 6: Electron Microscopy 1. The key...

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