Lecture 10

Lecture 10 - Lecture 10: Microbial Growth 1. Understand the...

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Lecture 10: Microbial Growth 1. Understand the methods used to measure growth of bacterial cultures (and what their limitations are) a. The pour plate method i. Put sample in empty plate ii. Pour on warm liquid agar iii. Swirl everything around iv. Agar solution is very hot and can kill the bacteria b. The spread plate method i. Small drop of bacteria culture placed in middle of plate ii. Hockey stick used to spread liquid evenly across top (like we did in lab) c. Ways to measure growth: i. Direct microscopic count—use a microscope to count each individual bacteria 1. Problem—can’t tell whether cell is dead or alive ii. Viable cell count—each viable cell results in a colony (we did this in lab 2— serial dilution) 1. Problem—we only know how to culture 1% of microorganisms in the world, it’s also very sensitive if plating conditions are optimal iii. Turbidity measurements—estimations of large number of bacteria in clear liquid media (by shining light through the suspended bacteria onto the photo-cell)
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This note was uploaded on 03/25/2010 for the course MB 351 taught by Professor Keen during the Spring '08 term at N.C. State.

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Lecture 10 - Lecture 10: Microbial Growth 1. Understand the...

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