2-25 - Antibody levels ( μ g/ml) IgA IgG IgM Intestinal...

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Unformatted text preview: Antibody levels ( μ g/ml) IgA IgG IgM Intestinal fuid 166 4 8 Colonic fuid 240-827 1 Trace amount IgA is the predominant immunoglobulin on mucosal surFace. Breast milk 470-12,340 40-168 6-18 Tears 80-400 Trace amount 0-18 Nasal fuid 70-846 8-304 Saliva 194-206 42 64 Transport oF IgA across mucosal epithelial cells Secretory IgA (sIgA) inhibits pathogen entry through mucosal surFace. sIgA is produced by B cells within mucosal tissues Healthy intestine villi in milk-fed neonatals milk formula Formula + IgA IgA prevents invasion of bacteria in intestine milk Formula + IgA formula Formula + IgG IgA in milk provides maternal protection for neonatals. Hematopoietic stem cells (HSC) Fluorescence-activated cell sorter (FACS) Flow cytometry is used to analyze cell surface markers. A + cells Red ¡uorescence Surface marker A Green ¡uorescence Surface marker B B + cells A- B- cells ¡orescent antibodies Cells can be separated according to cell surface markers. Negatively selected Positively selected Cells can be separated with antibodies coupled to magnetic beads. The purifcation oF HSC Bone marrow cells Magnetic beads-coupled antibodies against lineage markers (Lin) For diFFerentiated cells: B220 (B cells), CD4, CD8 (T cells), Mac-1, Gr-1 (myeloid cells: monocytes, granulocytes) Ter119 (erythrocytes) Magnetic separation Lin + cells (diFFerentiated cells) retained on column Lin- cells ¡ow through Cells incubated with ¡orescent antibodies against Sca-1, c-Kit, CD34 Separation by ¢ACS sorter CD34-/lo Sca-1 + c-Kit + Lin- cells 0.004% oF total bone marrow cells A Single HSC can reconstitute all the blood cells.A Single HSC can reconstitute all the blood cells....
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This note was uploaded on 03/25/2010 for the course BIO 360K taught by Professor Bryant during the Spring '09 term at University of Texas at Austin.

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2-25 - Antibody levels ( μ g/ml) IgA IgG IgM Intestinal...

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