Lab Practical 2 Outline

Lab Practical 2 Outline - Photosynthesis I Photosynthetic...

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Photosynthesis I - Photosynthetic autotrophs convert light energy into useful biological energy (ATP) and “reducing power” that drives the conversion of CO 2 into H 2 O and sugar. - Chlorophyll absorbs light energy. This permits the removal of electrons from water. Electrons and protons are shuttled so that ATP is generated and CO 2 is reduced. - Pigments absorb lights of particular wavelengths. - Absorption spectrum : Pattern of absorption by a substance - Steps: - Extract pigment from spinach leaves - Separate chlorophylls from cartenoids and then chlorophyll a from chlorophyll b - Determination of absorbance spectra - Spectrophotometer - measures light absorbance as a function of wavelengths - wavelength range: 400 to 700 nm - Carotene – ethanol - Chlorophyll a – petroleum ether - chlorophyll b – methanol - Carotene absorbs blue best - Chlorophylls a and b absorb blue and red best. They do not absorb any green. - Independent variable : wavelength - Dependent variable : absorbance Photosynthesis II - When chlorophyll absorbs energy, its electrons are excited to high energy levels. These
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Lab Practical 2 Outline - Photosynthesis I Photosynthetic...

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