principles and practices of agarose gel electrophoresis

principles and practices of agarose gel electrophoresis -...

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Table One: Distance Dye Traveled in Gel Dye How Much Moved (cm) Direction Attracted to A [orange] 3.8 + B [purple] 1.9 + C [red] 1.6 + D [blue 1] 0.6 + E [dye mixture] Separated into four 3.8; 1.9; 1.6; 0.6 +; +; + F [blue dye mixture] Separated into two 1.3; 1.9 +; - Sketch One: Distance Dye Traveled in Gel -2 -1 0 1 2 3 4 Distance (cm) A B C D E F Dye Graph One: Distance Dye Traveled in Gel 1st Color 2nd Color 3rd Color 4th Color
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Rachel Romm Biology 1H P5 Ms. Mackley 25 February 2009 Principles and Practice of Argose Gel Electrophoresis 1. On what basis does agarose gel electrophoresis separate molecules? Agarose gel electrophoresis are used to separate charged biomolecules such as DNA, RNA and proteins. 2. Explain migration according to charge. Molecules having a net negative charge migrate towards the positive electrode while net positively charged molecules migrate toward the negative electrode.
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Unformatted text preview: 3. What conclusion can be drawn from the results of sample E? Sample E is a mixture of dyes A, B, C, and D. 4. What conclusion can be drawn from the results of sample F? Sample F is does not contain dyes A, B, C, or D. 5. Why is glycerol loaded to the solution before they are loaded into the wells? Glycerol is loaded in order to give the mixture density. 6. What would happen if distilled water were substituted for buffer in either the chamber solution or the gel solution? The buffer solution contains electrodes so if it were to be substituted with distilled water there would be no electrodes. The dye is less dense than the buffer therefore; the dye sinks into the chamber. The buffer also serves as a conductor of electricity and controls the ph....
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This note was uploaded on 03/25/2010 for the course BIO 5381 taught by Professor Smith during the Spring '10 term at N. Arizona.

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principles and practices of agarose gel electrophoresis -...

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