lab4_hematology2007

lab4_hematology2007 - Department of Applied Biology and...

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ROSE-HULMAN INSTITUTE OF TECHNOLOGY Department of Applied Biology and Biomedical Engineering BE525 Biomedical Fluid Mechanics Lab 4: Hematology Lab K. Harbison and R. Bormann Objectives To become familiar with the technique for using a light microscope To see erythrocytes, leukocytes and thrombocytes under a microscope To learn to count blood cells using a hemocytometer To see blood cells resulting from one or two pathologies To learn to calculate the mean corpuscular volume of an erythrocyte Equipment Microscope Glass slide and cover slip sample of aseptic blood prepared blood smear slides from normal blood prepared blood slide showing pathologies hemocytometer capillary tubes and clay for plugging the tube microcentrifuge immersion oil 0.9 % NaCl solution for diluting pipette The blood which you will use for this lab is aseptic (sterile) animal blood purchased from Carolina Biological supplies. The blood is certified disease free, but any blood should always be treated as a potential hazard. Wear gloves, wash your hands after handling blood, and be especially careful not to expose any open cuts or sores to the blood. 1. Measure the hematocrit of the blood sample you obtain from the instructor. Fill a capillary tube. Spin in the microcentrifuge for 3 to 5 minutes. Calculate the hematocrit rather than simply reading it from the scale in the centrifuge. Is the hematocrit in the normal range? The Hematocrit measured for the A- blood provided came out to be 4%. This is extremely low, and does not match with the results found later in the lab concerning the amount of red blood cells per millimeter cubed. 2. Look at your blood sample under the microscope. Prepare a clean glass slide and clean cover-slip. Put one drop of blood on the slide. Cover with the cover slide. Look at this slide under the microscope using various magnifications. At the highest magnification, you will need to use immersion oil on the slide. How many erythrocytes should you see for each leukocyte? How many erythrocytes should you see for each platelet? Describe the shape of the erythrocytes. The leukocytes were hard to discern because they are transparent, and need a stain
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in order to view (their nucleuses). Also, the density of red blood cells was almost too overbearing. However, a few of what we thought looked like leukocytes could be seen. From a quick estimated count, we think we could see one leukocyte within a group of three hundred or so erythrocytes. Like the leukocytes, the thrombocytes/platelets were hard to see as well due to their small size. However, there seemed to be a considerable amount more platelets than leukocytes, one platelet per twenty or thirty erythrocytes to be more specific. The erythrocytes were disc-shaped with concave sides. 3.
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lab4_hematology2007 - Department of Applied Biology and...

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