2010 BICH 441 HW1

2010 BICH 441 HW1 - radioactive 32 P phosphate(P i is added...

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BIOCHEMISTRY 441 Spring 2010 Instructor: David O. Peterson Homework 1 Due in class Monday, Feb 08 1. As we will learn later in the semester, glucose can be stored in several tissues as a polymer called glycogen. Glucose monomers are released from glycogen by the action of glycogen phosphorylase, which catalyzes the reaction shown below, where the subscript on the glycogen indicates the number of glucose monomers in the glycogen polymer. glycogen n + P i glucose-1-P + glycogen n-1 The glucose-1-P can then be converted to glucose-6-P by the action of phosphoglucomutase, which does not require ATP. How much ATP would you expect to be generated during glycolysis using the glucose-1-P derived from the breakdown of glycogen? Explain your answer. (Express your answer in moles of ATP generated per mole of glucose-1-P.) 2. All the reactions of glycolysis can be shown to take place in crude cytoplasmic extracts of cells. If
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Unformatted text preview: radioactive ( 32 P) phosphate (P i ) is added to such extracts (along with glucose) and incubated for a short time, what glycolytic intermediate(s) (aside from ATP) would you expect to become labeled first? Would the result change if the incubation was allowed to proceed for a longer time? 3. ∆ G°' for the conversion of dihydroxyacetone-P to glyceraldehyde-3-P is +7.6 kJ/mol. If the intracellular concentrations of dihydroxyacetone-P and glyceraldehyde-3-P are 0.16 mM and 0.02 mM, respectively, what is ∆ G for this reaction inside the cell? What is K eq ' for this reaction? Use a calculator and show your work. (Assume the temperature is 25°C.) 4. Starting with glucose containing a 14 C label in the C2 carbon, draw the glycolytic intermediates from glucose to pyruvate showing the position of the labeled carbon in each molecule....
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