BIOCHEMISTRY 441 Spring 2010 Instructor: David O. Peterson Homework 3Due in class Mar 081. Using Table 20.1, calculate ∆Eo', ∆G°', and Keq' for the reaction catalyzed by isocitrate dehydrogenase as it occurs in the TCA cycle. 2. A. Mitochondria that are carefully isolated from other cellular components retain many normal mitochondrial functions. For example, isolated mitochondria can oxidize added pyruvate to CO2with corresponding reduction of O2and synthesis of ATP. A drug called Compound X has no effect on the ability of isolated mitochondria to oxidize pyruvate and consume O2, but X severely inhibits ATP synthesis. What does this tell you about the mechanism of action of Compound X? Explain your reasoning. B. A second drug, Compound Y, also severely inhibits ATP synthesis, but in the presence of Y, pyruvate oxidation and O2consumption are also inhibited. In the presence of both Compound X and Compound Y, ATP synthesis is inhibited, but pyruvate oxidation and O2
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