44110aHW3Ans

44110aHW3Ans - BIOCHEMISTRY 441 Spring 2010 Instructor:...

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BIOCHEMISTRY 441 Spring 2010 Instructor: David O. Peterson Homework 3 Due in class Mar 08 1. Using Table 20.1, calculate E o ', G°', and K eq ' for the reaction catalyzed by isocitrate dehydrogenase as it occurs in the TCA cycle. 2. A. Mitochondria that are carefully isolated from other cellular components retain many normal mitochondrial functions. For example, isolated mitochondria can oxidize added pyruvate to CO 2 with corresponding reduction of O 2 and synthesis of ATP. A drug called Compound X has no effect on the ability of isolated mitochondria to oxidize pyruvate and consume O 2 , but X severely inhibits ATP synthesis. What does this tell you about the mechanism of action of Compound X? Explain your reasoning. ANSWER : X is an uncoupler. It allows protons back into the matrix without generating any ATP. The electron transport chain is not affected because the proton gradient is being dissipated (just not in a useful way). B. A second drug, Compound Y, also severely inhibits ATP synthesis, but in the presence of Y, pyruvate
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This note was uploaded on 03/27/2010 for the course BICH 441 taught by Professor Staff during the Spring '08 term at Texas A&M.

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44110aHW3Ans - BIOCHEMISTRY 441 Spring 2010 Instructor:...

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