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PREPEX3 - Exam III Preparation Exam III will focus on...

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Exam III Preparation Exam III will focus on nitrogen metabolism, basically the material covered in Chapters 20 and 22. The topics begin with amino acid oxidation and end with the synthesis of nucleotides. Nitrogen is the theme for all of the reactions. It should be understood that the below list, while comprehensive over this section of the course, must not be considered complete. It is intended only as a study guide. Amino Acid Catabolism Start your studies by knowing how amino acids are prepared for metabolism. Know what is meant by a 26S proteosome, the 20S proteosome, the N-rule, reactions involved in protein ubiquination. Know the mechanism of an aminotransferase, the role of glutamate/ α -Kg pair in the reaction, the different forms and functions of vitamin B6 pyridoxal-PO 4 , pyridoxamine-PO 4 Be able to demonstrate a reaction involving an aminotransferase enzyme. Know the function of glutamate dehydrogenase, how the enzyme catalyzes the oxidative deamination of glutamate. Know how glutamine and glutamate contribute NH 3 to the urea cycle. Know urea cycle reactions (urea cycle tutorial will help), the amino acids, where ATP is needed in the cycle.
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