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506_507_quiz_3 - (b Phosphofructokinase(b(c Pyruvate...

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Name _____________________________ UIN ___________________ Biochemistry 411 Quiz 3 1.) (2 Points) What is the overall reaction of gluconeogenesis? Answer: 2 Pyruvate + 4 ATP + 2 GTP + 2NADH Glucose + 4 ADP + 2 GDP + 2 NAD + + 6Pi 2.) (4 Points) Gluconeogenesis is not simply the reverse of glycolysis. There are three irreversible steps of glycolysis that must be bypassed in order for gluconeogenesis to occur. The enzymes that catalyze the irreversible steps in glycolysis are shown below. For each of these steps, list the enzyme(s) that are used in gluconeogenesis to drive the formation of glucose from pyruvate. Glycolysis: Gluconeogenesis : (a.) Hexokinase (a.) ______________________________
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Unformatted text preview: (b.) Phosphofructokinase (b.) ______________________________ (c.) Pyruvate Kinase (c.) ______________________________ ______________________________ Answer (a.) Glucose-6-Phosphatase (b.) Fructose-1,6-bisphosphatase (c.) Pyruvate Carboxylase and PEP Carboxykinase 3.) (4 Points) Pyruvate Carboxylase has two active sites. Draw the mechanism for the reaction that occurs in the second active site: Pyruvate + CO 2 Oxaloacetate (Hint: The enzyme Pyruvate Carboxylase uses the cofactor biotin) NH N O S O O NH N O S CO 2 C O C H H H + CO 2 C O C H H O C O CO 2 C CH 2 O CO 2 Enz Enz NH NH O S + Pyruvate Biotin Oxaloacetate Enz...
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