NPB 128 Study Questions

NPB 128 Study Questions - NPB 128 Study Questions Set 1 1...

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NPB 128 Study Questions Set 1 1. Give three reasons to study comparative endocrinology 1. To help in biomedical research 2. To study the origins and evolutionary history of our endocrine system 3. To study the structure-function relationship of hormones and their receptors 4. To study how biological control and homeostasis 5. To study how animals adapt to the environment 2. You suspect that a hormone produced by a newly discovered gland controls a particular physiological process. What steps would you use to test your hypothesis? 1. Apply the hormone and contrast the effects with a control 1. Bioassay 1. x-axis = amount, y-axis = response 2. radioimmunoassay: measure concentration of antigen 3. ELISA: antibody is linked to an enzyme that catalyzes a signalling reaction 2. Remove the gland and compare/contrast effects with a control 3. Inject the hormone at different times and study different effects 4. Manipulate environment to induce hormone 5. Study the receptor as well as the effector gland and the target tissue 3. Give examples of endocrine or neuroendocrine glands that exist in fish but no other vertebrates. What hormones do they produce? Are these hormones made in mammals? Compare and contrast their roles in fish vs. mammals. 1. Urophysis 1. Urotensin 1 1. Blood pressure regulation 2. peptide 3. renin? 2. Urotensin 2 1. muscle relaxation 2. peptide 3. vasopressin? 3. Corpus of Stannius 1. paracrine autocrine 2. kidney and intestinal calcium absorption 3. no distinct gland in mammals 4. Recall our discussion of the radioimmunoassay. How would alternative splicing, amino acid differences between species, and different proteolytic processing in different cellls impact your interpretation of radioimmunoassay results for peptide hormones? (Important: antibodies only recognize small regions of proteins.) Think of reasons why a bioassay result and a radioimmunoassay result might conflict. 1. Radioimmunoassays rely on antibodies to attach to specific regions of antigens (the peptide hormone in this case). It studies hormones. Changing the peptide hormone would change the affinity of the hormone to the the antibody and therefore change the binding curve and our interpretation of the results. 2. Bioassay on the other hand, studies the response when you increase hormone level. So even if the peptide hormone sequence changed, its function remains the same and the effects of the bioassay remain unchanged.
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5. What is the advantage for vertebrates to have endocrine glands in addition to neurosecretory cells to produce hormones? 1. Allows us to have different levels of control. 2. Endocrine glands are slower but have more degrees of control. 3. Neuroendocrine are faster, less control, but more reflexive. 6.
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This note was uploaded on 03/28/2010 for the course NPB NPB 128 taught by Professor Furlow/chang during the Winter '10 term at UC Davis.

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NPB 128 Study Questions - NPB 128 Study Questions Set 1 1...

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