Biotech-Fall 2009

Biotech-Fall 2009 - B iotechnology Recombinant DNA...

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Recombinant DNA technology Clone- genetically identical strain or organism that originated from the same organism. cloning a gene -duplication of a gene isolated from an organism. ________________________________________________ 1. Techniques that are essential to biotechnology A. Restriction enzymes (Fig. 9.1, Table 9.1) -enzymes that cut DNA at a precise sequence. B. Gel electrophoresis (Fig. 9.2). Can separate different sized DNA by running through a gel matrix. 2. Applications for technology- genetic engineering using an engineered plasmid that can force a bacterium /yeast /plant to work for us. A. What is a plasmid vector? (Fig. 9.8) 1) Origin of replication 2) Multiple cloning site 3) Selectable marker 4) Secondary marker (optional)- insertional inactivation (Fig. 9.9) B. How do you decide which vector to use for your experiment (Fig. 9.6)? 1) Sometimes you want the DNA (gene you have inserted) to be replicated so you can use it in other applications (e.g. you clone the gene) (Fig. 9.3). 1
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This note was uploaded on 03/28/2010 for the course MCRO 251 taught by Professor Lorrainecramer during the Fall '09 term at UNC.

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Biotech-Fall 2009 - B iotechnology Recombinant DNA...

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