Handout _21-21A

Handout _21-21A - Bl8101 Handout #21 Reaction Catalyzed by...

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Unformatted text preview: Bl8101 Handout #21 Reaction Catalyzed by DNA Polymerase -0 cl) _O__ll=O ‘o—Tzo i ‘l Cl3H2 B1 CH2 B1 $5 ° 0 o I DNA Polymerase _ I ‘o—on , 0*]:20 ——-—-—> i ‘r (IJHz B2 CH2 82 O 0 9H T o 0‘ —O---P=O —-O—-Pl—-O-—ll3=0 (I) x \J I I 33 ' o~ ' 0 CH2 I 83 0 CH2 0 OH NTP 0“ O O *_ II II O—P—O—P—OH O O PPi, pyrophosphate BlSlOl HANDOUT 21A S. O’Neill Table 1. E. coli has three DNA polymerases. DNA Polymerase l DNA Polymerase ll DNA Polymerase lll Structure Daltons 109,000 90,000 ~900,000 Constitution Monomer Monomer Heteromultimeric ' Number/Cell 400 Not known 10-20 Enzymatic Activities 5’-3' elongation Yes Yes Yes 3’-5’ Yes Yes Yes exonuclease 5’-3’ Yes No No exonuclease Table 2. There are 5 mammalian DNA polymerases (identified by Greek letters; yeast equivalents are given Roman numerals). DNA a(l) 8(lll) e(ll) B y Polymerase Location Nuclear Nuclear Nuclear Nuclear Mitochondrial Function Lagging Leading Repair Repair Replication strand ’ strang synthesis & synthesis priming Relative ~80% 10-15% 2-‘15% activity Mass (daltons) 300,000 170- 250,000 40,000 180-300,000 230.000 Subunits . Catalytic Catalytic Catalytic Catalytic Catalytic core core core core 2 primase 1 unknown 1 unknown 2 unknown 1 unknown 3’-5’ No Yes Yes No Yes exonuclease ...
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Handout _21-21A - Bl8101 Handout #21 Reaction Catalyzed by...

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