CH369 exam1f98 - 1(6 pts Draw the functional group or...

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1. (6 pts) Draw the functional group or compound of the following organic compounds and name an amino acid that also contains the same group in its R side chain : a) ethanol b) acetic acid c) benzene 2. (3 pts) List 3 differences in prokaryotic and eukaryotic cells. 3. (6 pts) Two common laboratory buffers are MOPS and SHS. The nitrogen of the morpholino group of MOPS can be protonated (pKa=7.2) and the carboxyl group of the succinate of SHS can be ionized (pKa=5.5). a) What are the effective buffering ranges of MOPS and SHS? b) What are the ratios of basic to acidic species at pH 6.5? c) Which buffer would more effectively maintain the pH if acid were added to a pH 6.5 solution? Why?
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2 4. (13 pts) The dissociation of phosphoric acid yields 4 species and 3 pKa values (2.2, 7.2, 12.7). a) Draw all the species generated in a titration of phosphoric acid and indicate the pKa values. b) You want to make 100 ml of 0.1 M phosphate buffer, pH 7.6. You find in the refrigerator, 0.1 M Na 2 HPO 4 and 0.2 M NaHPO 4 . Indicate the amounts of ALL ingredients you would need to prepare this buffer.
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