CH369 exam1s98 - Exam I CH 369 An * indicates the question...

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1 Exam I February 12, 1998 CH 369 An * indicates the question was from Tuesday questions. 1. (6 pts) Name three biopolymers and the building blocks (a general name will be sufficient) used to make the polymer: a. ______________________ are made from _____________________ b._______________________ are made from _____________________ c._______________________ are made from ______________________ *2. (6 pts) Water makes up 60-80% of the a cell. Name the chemical properties that make water fundamental to the biochemistry of cells and explain why they are important. 3. (5 pts) You have isolated a new protein. You carryout a gel filtration experiment in 20 mM phosphate buffer, pH 7 and determine the molecular weight to be 360,000. You then run the same gel filtration experiment in the presence of 8 M urea and obtain a single peak with a molecular weight of 60,000. In a third experiment, you run the gel filtration in the presence of 8 M urea and 10 mM β -mercaptoethanol and obtain two peaks, 15,000 and 30,000. The peak height of the15,000 molecular weight polypeptide was twice as high as the 30,000 molecular weight polypeptide.
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CH369 exam1s98 - Exam I CH 369 An * indicates the question...

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