CH369 exam4f98 - Exam 4 December 5 1997 Name 1 a b c d e(20...

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1 Exam 4 December 5, 1997 Name_____________________________ 1. (20 pts) On the outline of electron transport below: a) at the entry points into electron transport indicate oxidation state and/or molecule b) fill in the missing components c) circle the mobile electron carriers d) indicate where the protein complexes function e) which steps generate sufficient energy to support ATP synthesis NAD + Fe ± cytb cytb Fe cytc 1 Cu cyta cyta 3 FAD Fe ² 2. (5 pts) Functional electron-transport systems can be reconstituted from purified respiratory electron-transport chain components and membrane particles. For each of the following sets of components, determine the final electron acceptor with given the given purified components . Assume O 2 is present. a) NADH, Q, complexes I, III and IV:_______________ b) NADH, Q, cytochrome c, complexes II and III:_________________ c) succinate, Q cytochrome c, complexes II, III, and IV:_____________ d) succinate, Q, cytochrome c, complexes II and III:________________ e) succinate, Q, complexes I and III:________________ 3. (8 pts) a) When the widely prescribed painkiller Demerol is added to a suspension of respiring mitochondria, the ratios of NADH/NAD+ and Q/QH 2 increase. Which electron- transport complex is inhibited by Demerol? b) When the antibiotic myxothiazole is added to respiring mitochondria, the ratios of cytc c 1 (Fe +3 )/cytc c 1 (Fe +2 ) and cytcb 566 (Fe +3 )/cytcb 566 (Fe +2 ) increase. Where does myxothiazole inhibit the electron-transport chain?
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2 c) The c subunits of the F 0 component of ATP synthatase form an ion channel across the
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This note was uploaded on 03/30/2010 for the course CH 369 taught by Professor Kbrowning during the Spring '07 term at University of Texas.

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CH369 exam4f98 - Exam 4 December 5 1997 Name 1 a b c d e(20...

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