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Unformatted text preview: Sarah Henthorn Group Members: Laura Soileau and Matt Sandoz Lab Topic: Enzyme Concentration Effects of Enzyme Concentration on Catecholase Activity Last week, our lab group decided to test the impact of enzyme concentration on an enzyme catalyzed reaction. The enzyme in this experiment was benzoquinone. The experimental setup consisted of three different treatments each tested three times in culture tubes to determine enzyme concentration. In the first treatment, the blank solution consisted of 8mL of deionized water pipetted into a culture tube along with 1mL of potato enzyme. This blank tube was used to set the spectrophotometer to zero. The spectrophotometer then was set to read absorbance. The three tubes of treatment one included 3mL of deionized water, 5mL of catechol, and 1mL of potato enzyme. These tubes were put into the spectrophotometer to record the absorbance for two minutes. We then recorded the data on a graph. For treatments two and three, the same thing was repeated in the use of the spectrophotometer; however...
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- Spring '08
- Null hypothesis, deionized water, potato enzyme, Sarah Henthorn