LM8 - Lectureoverview conjugation transformation...

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Lecture overview • Creating genetic diversity in microbes via  horizontal gene transfer  – conjugation – transformation  – transduction • Transposable elements (transposons) 1
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Creating genetic diversity via horizontal gene  transfer (Fig. 14.11) 2 • Conjugation – plasmid is  transferred that replicates  autonomously • Transformation – DNA is  taken up and incorporated via  homologous recombination • Transduction – phage carries  DNA that is incorporated into  genome via homologous  recombination • In all cases DNA may be  degraded if it is recognized as  foreign
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Bacterial conjugation (Fig. 14.19) • In Gram-negative cells it  depends on specialized  (conjugative) plasmids that  contain genes that cause the  transfer of the plasmid from  one cell into another (often  unrelated genetically) • Some plasmids contain genes  that result in transfer of  themselves from bacteria to  eukaryotic cells  • Ti (tumor-inducing) plasmid is  transferred from Agrobacteria  to plant cells 3
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Bacterial conjugation (Fig. 14.19) • Some R plasmids also transfer  antibiotic resistance genes • Many conjugative plasmids  carry virulence genes that  confer the ability to cause  disease • Some Gram-positive cells also 
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This note was uploaded on 03/31/2010 for the course MCB 3020 taught by Professor Ogden during the Spring '08 term at University of Florida.

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LM8 - Lectureoverview conjugation transformation...

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