LM12-1 - OverviewofMicrobialEcology

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Overview of Microbial Ecology • Microbial ecology is the study of the interactions of  microbes with their environments • There are currently 10 30  prokaryotes on earth • Microbes are metabolically active and change the  environment they live in Microbes responsible for levels of O 2 , CO 2  and N 2  in  atmosphere 1
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Studying microbes in the lab (Fig. 25.11) • Most microbes from  environment cannot be  cultivated (<<1%) • Enrichment cultures  (right), finding and using  conditions that select for  the microbes of interest,   can help in some cases • Caution: culturing often  changes phenotypes • Many microbes only grow  in communities 2
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Modern approaches to the study of  microbial ecology (Fig. 25.9)  • FISH (right) – fluorescence  in  situ  hybridization uses  hybridization of microbial  DNA/RNA to tagged NA probes • Immunofluorescence microscopy  uses tagged antibodies to identify 
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LM12-1 - OverviewofMicrobialEcology

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