Problem Set 5 2010

Problem Set 5 2010 - Problem Set 5 Chapters 16 - 19 BILD1 /...

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Problem Set 5 BILD1 / Winter 2010 Chapters 16 - 19 Traver 1 DNA 1) How did the use of the following radioisotopes – 35 S, 32 P and 15 N – help demonstrate that the genetic material was DNA, and that the Watson and Crick model of DNA was correct? 2) (a) Draw in schematic form the nucleotide dCTP. Point out the 5’ end and the 3’ end. (b) Draw out the reaction for the addition of a second base to this nucleotide. (c) What was the energy source for this reaction? (d) How does dCTP differ from CTP? 3) What is Chargaff’s rule and how did the Watson-Crick structure of DNA explain this rule? 4) You have a DNA strand with the following linear sequence of bases: 5’ – G G C C C A T G A C C T C T A G – 3’ (a) What is the sequence of the complementary strand? Above, draw an arrow in the direction that replication would occur for polymerization of the complementary strand. Here is another strand of DNA: 3’ – G G C C C A T G A C C T C T A G – 5’ (b) What’s the sequence of its complementary strand? Draw an arrow above in direction of replication.
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Problem Set 5 BILD1 / Winter 2010 Chapters 16 - 19 Traver 2 5) Draw a diagram of the DNA replication fork. Point out where the following things are likely located: (a) the leading strand
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This note was uploaded on 03/31/2010 for the course BIOLOGY BILD 1 taught by Professor Traver during the Winter '10 term at UCSD.

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Problem Set 5 2010 - Problem Set 5 Chapters 16 - 19 BILD1 /...

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