11 - Eukaryotic gene regulation Transcriptional control RNA...

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Unformatted text preview: Eukaryotic gene regulation Transcriptional control RNA Pol activity requires multiple subunits RNAP carboxy-terminal- domain (CTD) Control elements Reporter genes Txn activators Eukaryotic gene regulation Transcriptional control : Gene expression in eukaryotic cells regulated primarily by regulation of transcription Mechanisms to regulate transcription: Chromosomal states--condensed vs non-condensed Factors---RNA polymerases, activators, repressors Cis elements ! Promoter elements (TATA box, initiator, promoter-proximal elements) ! Transcription start site ! Enhancers Chromatin Remodeling Activators and repressors determine whether or not transcription will occur Repressors return chromatin to its condensed state Activators function to decondense chromatin Characterization of eukaryotic RNA polymerases In Eukaryotic cells, RNA Polymerase I RNA Polymerase II RNA polymerase III Biochemically, three distinct RNA pols were eluted at different salt concentrations during ion-exchange chromatography NaCl Cell extract RNA polymerase I: RNA synthesis occurred even with 10 mg/ml !-amanitin RNA polymerase II: no RNA synthesis with 1 mg/ml...
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11 - Eukaryotic gene regulation Transcriptional control RNA...

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