16 - Processing of rRNA and tRNA(p 358 364 1 Nucleolus 2...

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Processing of rRNA and tRNA (p. 358 - 364) 1. Nucleolus 2. 45S pre-rRNA 18S,5.8S, 28S 3. tRNA maturation Features of Different Eukaryotic RNAs: regulation 20 - 24 II miRNA splicing ~100 - 200 II - U1, U2, U4, U5 III - U6 snRNA translation ~75 - 80 III tRNA translation 5000 (28S) 1900 (18S) 150 (5.8S) 120 (5S) I - pre-rRNA III - 5S rRNA translated to protein variable II mRNA Type Pol Mature Size Function
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small ribosomal subunit “small” rRNA Large ribosomal subunit - contains 3 different rRNAs rRNA content of Ribosomes rRNA gene organization - similarities among all eukaryotes - genes in long tandem arrays separated by non-transcribed spacers - always arranged in same 5’ to 3’ order of 18S, 5.8S and 28S - pre-rRNAs genes code for transcribed spacers, which are removed “processome” - involved in processing pre-rRNAs Transcription unit Many RNA Pol I transcribing simultaneously
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www.dmb.rug.ac.be/u2/contents/courses/Files/Groep%205/Samenvatting.html Nulceolus : subnuclear “compartment” What happens in the Nucleolus: • RNA Pol I transcription • rRNA processing • Ribosome assembly
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