Eukaryotic Gene Regulation Review

Eukaryotic Gene Regulation Review - Libraries DNase Foot...

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Lec 11/12: Eukaryotic gene organization
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General Promoter Elements - TATA Box - Initiator Sequence - CpG Islands Enhancer Elements can be (1) can be far away from transcription start site (2) multiple - more than just one (3) can be located within introns or exons Types of Reg. Sequences
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Reg. Sequence Focused Experiments: Linker Scanning Mutations Vs. 5’ Deletion Assay
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T.F. Focused Experiments cDNA expression
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Unformatted text preview: Libraries DNase Foot Print assay EMSA And.S1 Nuclease Assay The Polymerases internal intermediate tRNA + 5s rRNA Pol III Upstream (usually TATA box) sensitive mRNA Pol II upstream resistant rRNA Pol I Promoter Element Location Amanitin sensitivity Product RNA:-All use TBP-Only pol II has CTD-Only pol II binds TATA box Transcription Initiation Activation and repression Mediators...
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Eukaryotic Gene Regulation Review - Libraries DNase Foot...

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