15 Lec 15 Traditional Methods

15 Lec 15 Traditional Methods - Rediscovering the original...

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Rediscovering the original methods of soil microbiology Catherine Osborne
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Beijerinck, the first environmental microbiologist 1888
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The microscopically- determined size of the soil bacterial population is at least 20 times greater than the plate counts from the same soils Conn, the first soil microbial ecologist 1918
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100% 5% “The Great Plate Count Anomaly” 1985
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The Ribosomal Revolution Carl Woese (1977) Norm Pace (1986)
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There are 9 major bacterial phyla in soil Proteobacteria Actinobacteria Firmicutes Bacteroidetes Acidobacteria Verrucomicrobia Chloroflexi Planctomycetes Gemmatimonadetes Commonly isolated and well studied Janssen (2006) AEM 72: 1719
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Soil substrate membrane system Ferrari et al. (2005) Appl Environ Microbiol 71: 8714
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Microcolonies on soil membranes Ferrari et al. (2005) Appl Environ Microbiol 71: 8714
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Increasing incubation time increases the plate count Janssen et al. (2002) Appl Environ Microbiol 68: 2391
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3 months, 25°C Dilute soil suspension Sieve Suspend in water Sonicate Sait et al . (2002) Environ Microbiol 4: 654
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Polymers may overcome substrate accelerated death Schoenborn et al. (2004) Appl Environ Microbiol 70: 4363
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Effect of medium and incubation time Davis et al . (2005) Appl Environ Microbiol 71: 826 “New” media
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15 Lec 15 Traditional Methods - Rediscovering the original...

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