Monday, October 12th, 2009

Monday, October 12th, 2009 - Monday, October 12th, 2009 I....

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Summary of malate prevision cost I. Tidy up in term of NAD (P) H II. Energy metabolism in the context of membrane associate electron transport III. Monomer - Polymerization - Transport - Precursor a. Fixed cost 46, 453 umole ATP = we get the sum cost for growing on malate, and what we should recognize is that these are fixed cost, irrespective to the cost of precursors = to get the final cost we add the precursor cost b. For growing on glucose, we had the gain in ATP = 40,860 umoles ATP c. Growing on malate, we have 53,984 umoles ATP = clearly cost more to grow on malate = costs 1.3 times more ATP to grow on malate than to grow on glucose = not only that, the number of how many umoles of Carbon are metabolized in making one gram of E coli = and in malate, there are twice as many number of carbon are required to make that one gram of E coli d. If E coli has the choice to grow on glucose or malate, if both of these are in the environment, E coli would chose to grow on glucose = there must be some mechanism in the cell to ensure that e. Growing on CO2? It's going to cost more = but how much more? f. Cost: 1. On glucose, we have generated 16,968 umoles of NADH = this NADH has to be oxidized = the cell has a certain amount of NAD and NADH = to keep central metabolism functioning, if all of the NAD is converted into NADH = everything stops ==> NADH has to be oxidized or excreted = this is a relatively expensive molecule = can't afford to excreting it = what the cell needs to do is to oxidize it a. Fermentation = when NADH is oxidized by pyruvate or a derivative of pyruvate = there is
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This note was uploaded on 04/02/2010 for the course MIC 140 taught by Professor Meeks,singer during the Spring '09 term at UC Davis.

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Monday, October 12th, 2009 - Monday, October 12th, 2009 I....

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