prelab12-mic102l-f-09

prelab12-mic102l-f-09 - density of the bacterial lawn. Why...

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MIC 102L Name: ___________________________ Prelab due at the beginning of Lab 12 1) What is a McFarland standard and how will you use it in Lab 12? 2) The table on page 104 of the lab manual lists the diameters of specific zones of inhibition (i.e. diameter of the area where the agar is clearer) expected for each antibiotic paired with each type of bacterium. (For penicillin, for example, resistant enterococci are able to grow closer to the disc than are resistant staphylococci, etc.) At the bottom of page 104 the lab manual indicates that for the results to match a real Kirby-Bauer antibiotic test, we should be incubating our bacterial/antibiotic combinations at 35 o C (we do 37 o C) in a plate of a special medium poured to a specific depth (we used standard NA plates). Two other factors involved in setting up the incubation that we do control correctly are potency of the antibiotic and
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Unformatted text preview: density of the bacterial lawn. Why do these two factors need to be controlled for an accurate test? 3) In Lab 12, you will be performing antibiotic testing on E. coli and Staphylococcus epidermidis . What is the temperature of incubation for each? How many days should each be incubated before being placed in the 4 o C room? Bacterium Incubation temperature? Duration of incubation? E. coli Staphylococcus epidermidis . 4) I used incubated / non-incubated hamburger in Lab 10 (circle one). On the selective and differential MacConkey Agar used with hamburger in lab 10, what do bright red colonies represent? Based on your own plate data for Lab 10, what was the abundance in the hamburger of the bacteria that produced a positive result on this agar?...
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This note was uploaded on 04/02/2010 for the course MIC 102L taught by Professor Igo/nelson during the Spring '09 term at UC Davis.

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