lecture-3-102l-fall-09

Lecture-3-102l-fall- - Lecture 3 Biochemical Identifications and World of 7 1 Final syllabus in lab tomorrow 2 Key to worksheet#1 posted soon 3

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Lecture 3 – Biochemical Identifications and World of 7 1) Final syllabus – in lab tomorrow 2) Key to worksheet #1 posted soon 3) World of 7 1) Biochemical testing 2) Your unknown 3) Dichotomous key 4) Confirmatory tests? 5) Basis for lab report #1 4) Warm & cold rooms stay open until 4 PM M-Th, Friday until 5 PM
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Lab Lecture Schedule: 12:10-1:00 PM Mondays; Room 226 Wellman Week Dates 1 September 28 Introduction to Microscopy 2 October 5 Bacterial growth, semi-log plots, doubling time 3 October 12 Biochemical Identification & World of 7 4 October 19 MPN, water quality testing and enrichments (part 1) 5 October 26 Food safety; microbial nutrition; enrichments (part 2) 6 November 2 MIDTERM 1 7 November 9 Microbial nutrition (part 2) – life on/in agar-gelled medium 8 November 16 Bacterial identification, classification, phylogenetic probes and FISH 9 November 23 Microbial identification: PCR, API, ELISA, etc. 10 November 30 Review including preparation of notebooks and Lab Report 2 Finals week December 7 3:30 to 5:30 MIDTERM 2 – includes concepts covered in MT1 (class is cumulative; review concepts missed on MT1)
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4 L6 – October 13 A: World of 7: biochemical & Gram stain characterization B : Work on microscopy and streaking practicals C. Bring laboratory notebook up to date L7 – October 15 A. Read biochemical tests for World of 7. B. Receive “unknown” from world of 7 (each student). Make wet mount and Gram stain. Then perform appropriate tests & inoculate appropriate medium as defined by Fig. 7.1 or 7.2. C. Last day to pass microscopy and streaking practicals D. Notebooks due 5 L8 – October 20 A: Make and pour nutrient agar plates. B: Water quality testing. Read MPN tubes after 24 hours! C: Read biochemical tests for unknown; set up confirmatory tests. D: Setting up LSL enrichment (one per student). E: Setting up class enrichments for lactic acid bacteria & benzoate degraders (2 or 3 per lab) L9 – October 22 A: Read MPN test & calculate MPN B: Finalize identification of “unknown” from World of 7. C: Streak an EMB plate from your LSL enrichment D: Get update on progress of two types of class enrichments 6 L10 – October 27 A: Viable cell count - hamburger experiment (hockey sticks) B: C: Streak separate NA plate with each colony type of soil enteric from EMB agar. D:
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This note was uploaded on 04/02/2010 for the course MIC 102L taught by Professor Igo/nelson during the Spring '09 term at UC Davis.

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Lecture-3-102l-fall- - Lecture 3 Biochemical Identifications and World of 7 1 Final syllabus in lab tomorrow 2 Key to worksheet#1 posted soon 3

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