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lablecture5 - Microbial Nutrition and Enrichments (part 2)...

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Microbial Nutrition and Enrichments (part 2) Lecture 5 (10-26-09 D. Nelson) Study Guide on website. Will discuss lab report here. Review sessions: Room 2098 SLB both days Thursday 4:00 to 6:00 PM Sunday noon to 2:00 PM –east facing side door will be unlocked
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Possible topics for the last two categories include but are not limited to : general lab safety; aseptic techniques; steps involved in focusing/aligning a compound microscope for phase contrast or brightfield microscopy; steps involved in Gram staining procedure and relating Gram positive vs. negative outcome to structure; common mistakes in Gram stain procedure and how to avoid or rule them out; how to score LSL broth for growth of presumptive coliform bacteria; coliform vs. enteric distinction; definition of MPN and how to determine it; magnification, resolution, limit of resolution; contrast, phase contrast; bacterial growth, “phases” of a bacterial growth curve, binary fission; arithmetic, logarithmic, and semi-log plots; impact of temperature, quantity of nutrients and quality of nutrients on growth rate; different methods for determining how many bacteria/microbes are present in a liquid culture; although you will not be asked to plot data given a graph of data you should be able to interpret it; difference between macronutrients micronutrients and growth factors and some examples; approximate elemental composition of bacteria for 6 most abundant elements (C, O, N, H, P, S); major macromolecules that comprise bacterial cells and which of the most abundant elements are present in each; defined vs. complex media; number of organic compounds required to establish a defined medium for a bacterium; selective vs. differential media; dual role for organic C in nutrition of chemoorganoheterotrophic bacteria. Be sure you know the full dichotomous key for the World of 7 (page 68-69 lab manual). Although the great majority of the exam will be objective (multiple choice, fill in blank, etc.) there may be 1-2 short answer questions related to: (a) basis for any of the 3 enrichments set up in lab 7; (b) the rationale for secondary enrichments, use of a small soil inoculum and alternating between selective and non-selective agars in trying to achieve a pure culture, which will be discussed in Monday Lecture #5.
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This note was uploaded on 04/02/2010 for the course MIC 102L taught by Professor Igo/nelson during the Spring '09 term at UC Davis.

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lablecture5 - Microbial Nutrition and Enrichments (part 2)...

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