lablecture4-f-09

lablecture4-f-09 - Media Composition and Enrichments (part...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Media Composition and Enrichments (part I) Selective and Differential Media MPN & Water Quality Testing Lecture 4 (10-19-09 D. Nelson) Calculating CFU and Percent Solutions docs on website, hopefully by end of day Worksheet 2 doc handed out here. Need to read 9 tubes 37 o C from Lab 8 at approx. 24 hours, i.e. Wednesday before 4PM. This is the presumptive multiple tube fermentation part of test for coliform bacteria. Either transport metal pan (or paper cup) from 37 o C room to lab bench or read in 37 o C room. PLEASE DONT READ MPN TUBES IN HALLWAY. Media List here. Water = 70=80% Natural microbial populations: Redfield ratio (molar): C:N:P 106:16:1 Fast growing E. coli cells: Table at left (molar): C:N:P 43:10:1 Not a single value but a fairly restricted range for C:N:P regardless of where growing. Know C 50% dry wt and also know C, O, N, H. P & S as most abundant macronutrients. These 6 elements > 95% dry wt. E. coli , balanced growth, 37oC, glucose minimal medium. Total weight of one cell = 9.5 x 10-13 g (0.95 pg) Water in cell (70% of total) = 6.7 x 10-13 g (0.67 pg) Dry weight of one cell = 2.8 x 10-13 g (0.28 pg) Macromolecule % of total dry wt Protein 55.0 RNA (r, t & m) 20.5 DNA 3.1 lipid 9.1 lipopolysaccharide 3.4 peptidoglycan 2.5 glycogen 2.5 Soluble pools 2.9 Inorganic ions 1.0 TOTAL 100.0% In Lab 4 we measured E. coli growth by determining optical density with a spectrophotometer. Other possible (and common*) ways to measure growth? *Total organic C *Total cellular protein *Total dry weight Total cellular S (1%, NOT commonly used) (*Direct cell counts via microscopy) Energy Source Source of cell carbon Organic CO 2 Chemical (organic or inorganic) Chemoorganoheterotroph (organic for energy, too) Every microbe in this course except Nostoc species....
View Full Document

This note was uploaded on 04/02/2010 for the course MIC 102L taught by Professor Igo/nelson during the Spring '09 term at UC Davis.

Page1 / 21

lablecture4-f-09 - Media Composition and Enrichments (part...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online