MCB164+MT1+S04+KEY

MCB164+MT1+S04+KEY - Name_KEY_ MCB164 Spring 2004 Page 1 of...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Name__________KEY_____________________ MCB164 Spring 2004 Page of 6 ID #________________________________ FIRST MIDTERM April 26, 2004 Question Points Score 1 15 2 12 3 18 4 10 5 27 6 12 7 6 Total 100 This examination is closed book. There are 6 pages to the exam. Please count them before you start to make sure all are present . Please write your name on each page of the exam . Answer each question in the space provided. If additional space is required use the back of the page and indicate clearly that you continued your answer on the back. Do not attach additional pages. GOOD LUCK! Student authorization: I authorize the instructor to return the graded exam to me in the main office or to distribute it in the bin for me to pick up. Signature _____________________________ Date_____________________ 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Name__________KEY_____________________ MCB164 Spring 2004 Page of 6 1. (15 points) List one advantage and one disadvantage for each of the following methods of mutagenesis: Possible answers are given. Other correct answers exist. A. EMS Advantages- 1. Can be used to create missense mutations that may affect a binding site or an active site of protein or enzyme. 2. May be used to find partial loss-of- function, or conditional alleles of essential genes. 3. EMS is easy to administer to organisms by soaking or feeding. Disadvantages-1. very dangerous to work with since it is a powerful mutagen. 2. Mutations caused by EMS are not linked to any marker except the phenotype they create. B. Transposon Advantages- 1. Mutations are marked genetically so that linkage of the mutant phenotype to the transposon marker can be followed during segregation analysis. 2. Genomic DNA flanking the point of insert can be cloned by plasmid rescue. 3. Useful for creating gene disruptions or null mutations. Disadvantages-1. Transposition events have limited target specificity so some gene
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 6

MCB164+MT1+S04+KEY - Name_KEY_ MCB164 Spring 2004 Page 1 of...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online