MCB164+MT2+S09+key

MCB164+MT2+S09+key - Name_ MCB164 Spring 2009 Page of 8 1...

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Name_______________________________ MCB164 Spring 2009 Page of 8 SECOND MIDTERM May 19, 2009 Question Points Score 1 32 2 5 3 8 4 8 5 20 6 20 7 8 8 20 9 12 10 17 Total 150 This examination is closed book Please write your name on each page of the exam . There may be more than one correct answer for a given problem. You only need to provide one answer. HAVE FUN! 1
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Name_______________________________ MCB164 Spring 2009 Page of 8 1. (32 points) After years of study you have become a leading cancer researcher in your field. In your present position, you have access to hundreds of tumor tissues surgically removed from patients with breast cancer. A number of these women chose total mastectomy (breast removal) so you also have access to surrounding healthy tissue. A. (5) Your first line of study is to determine the degree of genomic instability in tumor cells of these patients. You want to know if the cells have the normal number of chromosomes (46). Briefly describe ONE methodology you would use to determine chromosome number in tumor cells. Isolate spread metaphase chromosomes; Perform karyotype by staining chromosomes with DAPI or FISH using chromosome-specific probes; Can also use antibody to CENP-A for immunofluorescence. B. (5) If you were to analyze the number of chromosomes in tumor cells from different patients do you expect these numbers would be the same or different? Explain briefly. The chromosome number would be different because the pattern of genomic instability will be unique to each tumor. C. (4) Next you want to measure whether or not telomerease is active in the tumor cells. You are able to measure telomerase activity in both tumor cells and healthy tissue from the same patient. Your expectation is that telomerease will be ( ACTIVE / INACTIVE) in tumor cells and (ACTIVE / INACTIVE ) in healthy tissue. (circle one word in each case) D. (6) You interview the women to determine those with high incidence of breast cancer among their immediate family members. Draw a simple pedigree showing autosomal dominant inheritance. Include three generation. Use boxes for men, circles for women and filled in circles for affected women. Assume complete penetrance of the phenotype. Various E. (4) From your analysis you conclude that about 10% of the individuals are from families that carry a mutation in a ( TUMOR SUPRESSOR GENE / ONCOGENE). 2
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Name_______________________________ MCB164 Spring 2009 Page of 8 F. (8) You have developed a simple diagnostic test to distinguish wild type, gain-of-function and loss-of-function mutations of a candidate gene mutated in the families . Your test also determines including copy number for each type. You apply this test to both healthy tissue and tumor tissue from affected women. What genotype(s) would you expect to observe in the paired tissues? Include the copy number of each allele. Assume that any difference in copy number is not attributed to genome instability. 1. TUMOR:
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MCB164+MT2+S09+key - Name_ MCB164 Spring 2009 Page of 8 1...

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