MCB164+S08+HW2+key - KEY MCB164 S08 Homework 2 Due in class...

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KEY MCB164 S08 Homework 2 Due in class may 29, 2008 Read the article referenced below and answer the questions that follow. Your answers should be typed. To receive full credit your answers must be clear and concise. PLoS Genet. 2007 Dec;3(12):e228. Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination. Alvaro D, Lisby M, Rothstein R. Department of Genetics and Development, Columbia University Medical Center, New York, New York, USA. To investigate the DNA damage response, we undertook a genome-wide study in Saccharomyces cerevisiae and identified 86 gene deletions that lead to increased levels of spontaneous Rad52 foci in proliferating diploid cells. More than half of the genes are conserved across species ranging from yeast to humans. Along with genes involved in DNA replication, repair, and chromatin remodeling, we found 22 previously uncharacterized open reading frames. Analysis of recombination rates and synthetic genetic interactions with rad52 suggests that multiple mechanisms are responsible for elevated levels of spontaneous Rad52 foci, including increased production of recombinogenic lesions, sister chromatid recombination defects, and improper focus assembly/disassembly. Our cell biological approach demonstrates the diversity of processes that converge on homologous recombination, protect against spontaneous DNA damage, and facilitate efficient repair.
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This note was uploaded on 04/04/2010 for the course MCB 164 taught by Professor Burgess during the Spring '08 term at UC Davis.

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MCB164+S08+HW2+key - KEY MCB164 S08 Homework 2 Due in class...

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